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Applied Biosystems offers the gold
standard for high performance, multicolor
real-time PCR. With our real-time PCR
platforms you get the convenience of
universal cycling conditions with TaqMan®
assays and the flexibility of unlimited
real-time chemistry choices.
- 7500 Fast Real-Time PCR System
Enables standard 96-well-format high-speed thermal cycling, delivering results in less than
30 minutes.
- 7500 Real-Time PCR System
Gives you even more—including enhanced dye flexibility.
- 7300 Real-Time PCR System
Combines high-end results with exceptional budget sensitivity.
Whatever solution you choose, it is always real easy with Applied Biosystems TaqMan® assays, reagents, and consumables.
The Applied Biosystems HRM Advantage:
- Does not require temperature shift-easily distinguish between homozygous
mutant and wild type samples
- Shortens analysis time by auto-calling genotypes and automatically
omitting the no template controls
- Minimizes subjective analysis by automatically grouping unknown variant
clusters
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| Figure 1 Difference plot generated with the Applied Biosystems HRM application on the 7500 Fast |
Figure 2 Difference plot generated on another plate based real-time HRM system |
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| The ability to easily identify new variants is key for successful HRM applications. When comparing Applied Biosystems' HRM application on the 7500 Fast Real-Time PCR System to another plate based real-time HRM system, the ability of the AB HRM application to better identify homozygous mutant samples was clear. By eliminating the temperature shift step, the AB HRM solution was able to clearly distinguish homozygous mutant samples from homozygous wildtype samples in 97.5% of the population, whereas the other HRM system was only able to distinguish them in 10% of population. All genotypes were auto-called by the respective software packages and were not altered by the operator.
Class 1 SNP (A/G), multiple technical replicates of 9 DNA samples representing three genotypes: homozygous wildtype (G/G), homozygous mutant (A/A) and heterozygous (A/G)
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For Research Use Only. Not for use in diagnostic procedures.
Practice of the patented polymerase chain reaction (PCR) process requires a license. These systems are Authorized Thermal Cyclers for PCR and may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR license in accordance with the label rights accompanying such reagents.
Purchase of this instrument does not convey any right to practice the 5' nuclease assay or any of the other real-time methods covered by patents owned by Roche or Applied Biosystems
Applied Biosystems is a registered trademark and AB (Design), Applera, iScience and iScience (Design) are trademarks of Applera Corporation or its subsidiaries in the US and/or certain other countries. |
